H. Filsoofiana,b, S. Akbarib, A. Akbaria*
a GreenNanoTech Kft, Király Utca 80, Budapest, 1068, Hungary
b NanoSciTec GmbH, Hermann Weinhauser str. 67, Munich, 81867, Germany
Abstract
| Reduced erythrocyte deformability is one of the important causes for stroke and heart failure in hypertensive patients. Deformability is a biophysical feature of erythrocyte determined by membrane cortex structure. Proteins of membrane skeleton such as spectrin, actin, band 4.1, ankryn and transmembrane protein Band 3 play a more important role than other membrane components in determining erythrocyte deformability. However, changes in proteins of erythrocyte membrane skeleton in hypertension have not been investigated so far. Therefore, in this report, calorimetric and spectroscopic investigations of the proteins of erythrocyte membrane skeleton are investigated in hypertensive rats and normal animals. Several methods were applied including differential scanning calorimetry (DSC), circular dichroism spectropolarimeter (CD), fourier transform infrared (FTIR). Our results showed that proteins of erythrocyte membrane skeleton, especially spectrin and Band 3 protein in hypertensive rats, are more compact than in normal rats. These changes are likely to have considerable effect on the membrane phospholipids and will increase their rigidity, which will ultimately reduce erythrocyte deformability. Keywords: hypertension, deformability of erythrocyte, protein of membrane skeleton, CD, DSC © Article info: Accepted by: 14 November 2021, Published by: 1 December 2021. |
*Corresponding author: A. Akbari. Tel.: +36-20-453-7574
E-mail address: armita.akbari@greennanotec.com
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Experimental evaluation of erythrocyte proteins membrane skeleton in
hypertension
